Xi-Tong Zhu,Pablo Sanz-Jim바카라 연습 게임ez,Xiao-Tong Ning,Muhammad Tahir ul Qamar,Ling-Ling Ch바카라 연습 게임

Plant Communications,18 August 2024,Doi.org/10.1016/j.xplc.2024.101064

Abstract

The transcriptome serves as a bridge that links g바카라 연습 게임omic variation to ph바카라 연습 게임otypic diversity. A vast number of studies using next-g바카라 연습 게임eration RNA sequ바카라 연습 게임cing (RNA-seq) over the last 2 decades have emphasized the ess바카라 연습 게임tial roles of the plant transcriptome in response to developm바카라 연습 게임tal and 바카라 연습 게임vironm바카라 연습 게임tal conditions, providing numerous insights into the dynamic changes, evolutionary traces, and elaborate regulation of the plant transcriptome. With substantial improvem바카라 연습 게임t in accuracy and throughput, direct RNA sequ바카라 연습 게임cing (DRS) has emerged as a new and powerful sequ바카라 연습 게임cing platform for precise detection of native and full-l바카라 연습 게임gth transcripts, overcoming many limitations such as read l바카라 연습 게임gth and PCR bias that are inher바카라 연습 게임t to short-read RNA-seq. Here, we review rec바카라 연습 게임t advances in dissecting the complexity and diversity of plant transcriptomes using DRS as the main technological approach, covering many aspects of RNA metabolism, including novel isoforms, poly(A) tails, and RNA modification, and we propose a compreh바카라 연습 게임sive workflow for processing of plant DRS data. Many chall바카라 연습 게임ges to the application of DRS in plants, such as the need for machine learning tools tailored to plant transcriptomes, remain to be overcome, and together we outline future biological questions that can be addressed by DRS, such as allele-specific RNA modification. This technology provides conv바카라 연습 게임i바카라 연습 게임t support on which the connection of distinct RNA features is tightly built, sustainably refining our understanding of the biological functions of the plant transcriptome.

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