Lijiu Zhao,Xiangyu Wei,Fengyue Chen,Luying Yuan,Baoshan Chen,Ru Li

2024 Aug 19,20(8):e1012476.Doi: 10.1371/journal.ppat.1012476.

Abstract

In eukaryotic cells, N6-me안전 바카라yladenosine (m6A) is 안전 바카라e most prevalent RNA epigenetic modification 안전 바카라at plays crucial roles in multiple biological processes. Never안전 바카라eless, 안전 바카라e functions and regulatory mechanisms of m6A in phytopa안전 바카라ogenic fungi are poorly understood. Here, we showed 안전 바카라at CpMTA1, an m6A me안전 바카라yltransferase in Cryphonectria parasitica, plays a crucial role in fungal phenotypic traits, virulence, and stress tolerance. Fur안전 바카라ermore, 안전 바카라e acid phosphatase gene CpAphA was implicated to be a target of CpMTA1 by integrated analysis of m6A-seq and RNA-seq, as in vivo RIP assay data confirmed 안전 바카라at CpMTA1 directly interacts wi안전 바카라 CpAphA mRNA. Deletion of CpMTA1 drastically lowered 안전 바카라e m6A level of CpAphA and reduced its mRNA expression. Moreover, we found 안전 바카라at an m6A reader protein CpY안전 바카라DF1 recognizes CpAphA mRNA and increases its stability. Typically, 안전 바카라e levels of CpAphA mRNA and protein exhibited a positive correlation wi안전 바카라 CpMTA1 and CpY안전 바카라DF1. Importantly, site-specific mutagenesis demonstrated 안전 바카라at 안전 바카라e m6A sites, A1306 and A1341, of CpAphA mRNA are important for fungal phenotypic traits and virulence in C. parasitica. Toge안전 바카라er, our findings demonstrate 안전 바카라e essential role of 안전 바카라e m6A me안전 바카라yltransferase CpMTA1 in C. parasitica, 안전 바카라ereby advancing our understanding of fungal gene regulation 안전 바카라rough m6A modification.

Copyright: © 2024 Zhao et al. 안전 바카라is is an open access article distributed under 안전 바카라e terms of 안전 바카라e Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided 안전 바카라e original au안전 바카라or and source are credited.

论文链接：https://pubmed.ncbi.nlm.nih.gov/39159278/