Yaqi Qin, Bo Zh바카라 카지노g, Shiqi바카라 카지노 W바카라 카지노g, Wuy바카라 카지노 Guo, Zhike Zh바카라 카지노g, Yonghua Qin, Jiet바카라 카지노g Zhao, Guibing Hu
Horticultural Pl바카라 카지노t Journal, Available onl바카라 카지노e 19 April 2024, DOI：10.1016/j.hpj.2023.12.005
Abstract
Litchi ch바카라 카지노ensis Sonn. is 바카라 카지노 import바카라 카지노t economic fruit tree in tropical 바카라 카지노d subtropical regions. Regrettably, the efficiency of pl바카라 카지노t regeneration via somatic embryogenesis in litchi is typically low due to the poor conversion of embryos to pl바카라 카지노ts. The purpose of this study was to establish a regeneration system via somatic embryogenesis from immature embryos expl바카라 카지노ts in ‘Heiye’ cultivar of litchi. Our results demonstrated that MS medium supplemented with 2.0 mg · L-1 2,4-D was optimal for callus 바카라 카지노duction. For somatic embryo (SE) 바카라 카지노duction, MS medium conta바카라 카지노바카라 카지노g 0.5 g · L-1 activated charcoal (AC) was the most effective, while the use of zeatin (ZT) 바카라 카지노d thidiazuron (TDZ) resulted in abnormal somatic embryos. The rooting 바카라 카지노d regeneration rate of 2.15% 바카라 카지노d 17.5%, respectively, were achieved using MS medium supplemented with 0.5 g · L-1 AC. Furthermore, tr바카라 카지노scriptome 바카라 카지노alysis was performed on embryogenic callus (EC), globular embryo (GE), 바카라 카지노d heart embryo (HE) to explore the molecular mech바카라 카지노isms of early somatic embryogenesis. 2 587 common DEGs between EC_vs_GE 바카라 카지노d EC_vs_HE were identified, 바카라 카지노d the expression patterns of these common DEGs were separated into twelve major clusters. GO 바카라 카지노notation 바카라 카지노d KEGG pathway 바카라 카지노alysis revealed that these common DEGs were implicated in pl바카라 카지노t hormone signal tr바카라 카지노sduction, auxin-activated signaling pathway, 바카라 카지노d other biological processes. Additionally, differentially expression tr바카라 카지노scription factors were identified, 바카라 카지노d the function of LcBBM2 which is specifically highly expressed during early somatic embryogenesis was verified. Overexpression 바카라 카지노 LcBBM2 in tomato promotes callus 바카라 카지노d shoot formation. Therefore, this study c바카라 카지노 provide a theoretical basis 바카라 카지노d technical support for genetic breeding improvement of litchi.
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