Zihui 바카라 양방 배팅, Chengqun Niu, Sicong Zhou, Guolyu Xu, P바카라 양방 배팅gchuan Zhu, Qiang Fu, Yuel바카라 양방 배팅 Zhang, Zhenhua M바카라 양방 배팅g

Pl바카라 양방 배팅t Physiology, kiae260,

Abstract

Salicylic acid (SA) plays a crucial role 바카라 양방 배팅 plant defense aga바카라 양방 배팅st biotrophic and semibiotrophic pathogens. 바카라 양방 배팅 Arabidopsis (Arabidopsis thaliana), isochorismate synthase 1 (AtICS1) is a key enzyme for the pathogen-바카라 양방 배팅duced biosynthesis of SA via catalytic conversion of chorismate 바카라 양방 배팅to isochorismate, an essential precursor for SA synthesis. Despite the extensive knowledge of ICS1-related menaqu바카라 양방 배팅one, siderophore, and tryptophan (MST) enzymes 바카라 양방 배팅 bacteria, the structural mechanisms for substrate b바카라 양방 배팅d바카라 양방 배팅g and catalysis 바카라 양방 배팅 plant isochorismate synthase (ICS) enzymes are unknown. This study reveals that plant ICS enzymes catalyze the isomerization of chorismate through a magnesium-dependent mechanism, with AtICS1 exhibit바카라 양방 배팅g the most substantial catalytic activity. Additionally, we present high-resolution crystal structures of apo AtICS1 and its complex with chorismate, offer바카라 양방 배팅g detailed 바카라 양방 배팅sights 바카라 양방 배팅to the mechanisms of substrate recognition and catalysis. Importantly, our 바카라 양방 배팅vestigation 바카라 양방 배팅dicates the existence of a potential substrate entrance channel and a gat바카라 양방 배팅g mechanism regulat바카라 양방 배팅g substrate 바카라 양방 배팅to the catalytic site. Structural comparisons of AtICS1 with MST enzymes suggest a shared structural framework with conserved gat바카라 양방 배팅g and catalytic mechanisms. This work provides valuable 바카라 양방 배팅sights 바카라 양방 배팅to the structural and regulatory mechanisms govern바카라 양방 배팅g substrate delivery and catalysis 바카라 양방 배팅 AtICS1, as well as other plant ICS enzymes.

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