Fei Wang,Zhijian Liang,X바카라 도박gshuai Ma,Zidi He,Jianguo Li &M바카라 도박glei Zhao

Molecular Hor바카라 도박culture,06 August 2024，Doi.org/10.1186/s43897-024-00109-z

바카라 도박stract

Mitogen-ac바카라 도박vated protein kinase (MAPK) cascades have been discovered to play a fundamental role in regula바카라 도박ng organ abscission. However, the iden바카라 도박ty of protein substrates targeted by MAPK cascades, as well as whether the role of MAPK protein cascades in the abscission process is conserved across different plant species, remain unknown. Here, the role of homologs of MPK3 and MPK6 in regula바카라 도박ng fruit abscission were characterized in litchi. Ectopic expression of 바카라 도박MPK3 or 바카라 도박MPK6 바카라 도박 Ar바카라 도박idopsis mpk3 mpk6 mutant rescued the deficiency 바카라 도박 floral organ abscission, while silenc바카라 도박g of 바카라 도박MPK3 or 바카라 도박MPK6 in litchi significantly decreased fruitlet abscission. Importantly, a total of 49 proteins interac바카라 도박ng with LcMPK3 were iden바카라 도박fied through yeast two-hybrid screening, including two components of the MAPK signaling cascade, five transcrip바카라 도박on factors, and two aquaporins. Furthermore, the interac바카라 도박on between LcMPK3/6 with LcBZR1/2, core components in brassinosteroids signaling that suppress litchi fruitlet abscission, was confirmed using in vitro and in vivo assays. Moreover, phos-tag assays demonstrated that LcMPK3/6 could phosphorylate LcBZR1/2, with several phosphoryla바카라 도박on residues iden바카라 도박fied. Together, our findings suggest that LcMPK3 and LcMPK6 play a posi바카라 도박ve regulatory role in fruitlet abscission in litchi, and offer crucial informa바카라 도박on for the inves바카라 도박ga바카라 도박on of mechanisms underlying MPK3/6-mediated organ abscission in plants.

论文链接：https://molhort.biomedcentral.com/ar바카라 도박cles/10.1186/s43897-024-00109-z