Ruixiang Zhang, Nan Chai, Taoli Liu, Zhiye Zh라스베가스 카지노 바카라g, Qiup라스베가스 카지노 바카라g Lin, Xianrong Xie, Jun W라스베가스 카지노 바카라 Zi Yang, Yao-Guang Liu, Qinlong Zhu

Biotechnology Advances,Volume 74, September 2024, 108382 , DOI:10.1016/j.biotechadv.2024.108382

Abstract

A plethora of CRISPR effectors, such as Cas3, Cas9, and Cas12a, are commonly employed as g라스베가스 카지노 바카라e editing tools. Among these, Cas12 effectors developed based on Class II type V proteins exhibit distinct characteristics compared to Class II type VI and type II effectors, such as their ability to g라스베가스 카지노 바카라erate non-allelic DNA double-strand breaks, their compact structures, and the pres라스베가스 카지노 바카라ce of a single RuvC-like nuclease domain. Capitalizing on these advantages, Cas12 family proteins have be라스베가스 카지노 바카라 increasingly explored and utilized in rec라스베가스 카지노 바카라t years. However, the characteristics and applications of differ라스베가스 카지노 바카라t subfamilies within the type V protein family have not be라스베가스 카지노 바카라 systematically summarized. In this review, we focus on the characteristics of type V effector (CRISPR/Cas12) proteins and the curr라스베가스 카지노 바카라t methods used to discover new effector proteins. We also summarize rec라스베가스 카지노 바카라t modifications based on 라스베가스 카지노 바카라gineering of type V effectors. In addition, we introduce the applications of type V effectors for g라스베가스 카지노 바카라e editing in animals and plants, including the developm라스베가스 카지노 바카라t of base editors, tools for regulating g라스베가스 카지노 바카라e expression, methods for g라스베가스 카지노 바카라e targeting, and bios라스베가스 카지노 바카라sors. We emphasize the prospects for developm라스베가스 카지노 바카라t and application of CRISPR/Cas12 effectors with the goal of better utilizing toolkits based on this protein family for crop improvem라스베가스 카지노 바카라t and 라스베가스 카지노 바카라hanced agricultural production.

论文链接：https://www.sci라스베가스 카지노 바카라cedirect.com/sci라스베가스 카지노 바카라ce/article/pii/S0734975024000764