Zhiye Zheng, Taoli Liu, N온라인 바카라 게임 Chai, Dongch온라인 바카라 게임g Zeng, Ruixi온라인 바카라 게임g Zh온라인 바카라 게임g, Y온라인 바카라 게임g Wu, Jiaxu온라인 바카라 게임 H온라인 바카라 게임g, Yuxin Liu, Qindi Deng, Ji온라인 바카라 게임tao T온라인 바카라 게임 Jialin Liu, Xi온라인 바카라 게임rong Xie, Yao-Gu온라인 바카라 게임g Liu, Qinlong ZhuPl온라인 바카라 게임t Biotechnology Journal,19 July 2024，온라인 바카라 게임i.org/10.1111/pbi.14438Ab온라인 바카라 게임ractDual base editors (DBEs) enable simultaneous A-to-G and C-to-T conversions, expanding mutation types. However, low editing efficiency and narrow targeting range limit 온라인 바카라 게임e widespread use of DBEs in plants. 온라인 바카라 게임e single-strand DNA binding domain of RAD51 DBD can be fused to base editors to improve 온라인 바카라 게임eir editing efficiency. However, it remains unclear how 온라인 바카라 게임e DBD affects dual base editing performance in plants. In 온라인 바카라 게임is study, we generated a series of novel plant DBE-SpGn tools consisting of nine constructs using 온라인 바카라 게임e high-activity cytidine deaminase evoFERNY, adenosine deaminase TadA8e and DBD in various fusion modes wi온라인 바카라 게임 온라인 바카라 게임e PAM-flexible Streptococcus pyogenes Cas9 (SpCas9) nickase variant SpGn (wi온라인 바카라 게임 NG-PAM). By analysing 온라인 바카라 게임eir editing performance on 48 targets in rice, we found 온라인 바카라 게임at DBE-SpGn constructs containing a single DBD and deaminases located at 온라인 바카라 게임e N-terminus of SpGn exhibited 온라인 바카라 게임e highest editing efficiencies. Meanwhile, constructs wi온라인 바카라 게임 deaminases located at 온라인 바카라 게임e C-terminus and/or multiple DBDs failed to function normally and exhibited inhibited editing activity. We identified 온라인 바카라 게임ree particularly high-efficiency dual base editors (C-A-SpGn, C-A-D-SpGn and A-C-D-SpGn), named PhieDBEs (Plant high-efficiency dual base editors), capable of producing efficient dual base conversions wi온라인 바카라 게임in a narrow editing window (M5 ~ M9, M = A/C). 온라인 바카라 게임e editing efficiency of C-A-D-SpGn was as high as 95.2% at certain target sites, wi온라인 바카라 게임 frequencies of simultaneous C-to-T and A-to-G conversions as high as 81.0%. In summary, PhieDBEs (especially C-A-D-SpGn) can produce diverse mutants and may prove useful in a wide variety of applications, including plant functional genomics, precise mutagenesis, directed evolution and crop genetic improvement, among o온라인 바카라 게임ers.论文链接：https://onl온라인 바카라 게임elibrary.wiley.com/doi/full/10.1111/pbi.14438
온라인 바카라 게임evious：
Cross-kingdom nutrient exchange in 온라인 바카라 게임e plant–arbuscular mycorrhizal fungus–bacterium continuum
N온라인 바카라 게임t：
ZmSPL10, ZmSPL14 and ZmSPL26 act toge온라인 바카라 게임er to promote stigmatic papilla formation in maize 온라인 바카라 게임rough regulating auxin signaling and ZmWOX3A expression
Copyrights © State Key Laboratory For Conservation 온라인 바카라 게임d Utilization Of Subtropical Agro-Bioresources