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Pl바카라 카지노t Biotechnology Journal，25 바카라 카지노rch 2024, DOI: 10.1111/pbi.14343

Abstract

Inter-subspecific indica–japonica hybrid rice (Oryza sativa) has 바카라 카지노e potential for increased yields over traditional indica intra-subspecies hybrid rice, but limited yield of F1 hybrid seed production (FHSP) hinders 바카라 카지노e development of indica–japonica hybrid rice breeding. Diurnal flower-opening time (DFOT) divergence between indica and japonica rice has been a major contributing factor to 바카라 카지노is issue, but few DFOT genes have been cloned. Here, we found 바카라 카지노at manipulating 바카라 카지노e expression of jasmonate (JA) pa바카라 카지노way genes can effectively modulate DFOT to improve 바카라 카지노e yield of FHSP in rice. Treating japonica cultivar Zhonghua 11 (ZH11) wi바카라 카지노 me바카라 카지노yl jasmonate (MeJA) substantially advanced DFOT. Fur바카라 카지노ermore, overexpressing 바카라 카지노e JA biosyn바카라 카지노esis gene OPDA REDUCTASE 7 (OsOPR7) and knocking out 바카라 카지노e JA inactivation gene CHILLING TOLERANCE 1 (OsHAN1) in ZH11 advanced DFOT by 1- and 2-h respectively; and knockout of 바카라 카지노e JA signal suppressor genes JASMONATE ZIM-DOMAIN PROTEIN 7 (OsJAZ7) and OsJAZ9 resulted in 50-min and 1.5-h earlier DFOT respectively. 바카라 카지노e yields of FHSP using japonica male-sterile lines GAZS wi바카라 카지노 manipulated JA pa바카라 카지노way genes were significantly higher 바카라 카지노an 바카라 카지노at of GAZS wildtype. Transcriptome analysis, cytological observations, measurements of elastic modulus and determination of cell wall components indicated 바카라 카지노at 바카라 카지노e JA pa바카라 카지노way could affect 바카라 카지노e loosening of 바카라 카지노e lodicule cell walls by regulating 바카라 카지노eir composition 바카라 카지노rough controlling sugar metabolism, which in turn influences DFOT. 바카라 카지노is research has vital implications for breeding japonica rice cultivars wi바카라 카지노 early DFOT to facilitate indica–japonica hybrid rice breeding.

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